Composite

Part:BBa_K2598025:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-09-25)


lacZ under CGG-regulated promoter pCGG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3112
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The subparts are provided by Christopher A voigt and assembled by us via Gibson Assembly.

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)

Macgregor, G. R., Nolan, G. P., Fiering, S., Roederer, M. & Herzenberg, L. A. Use of Escherichia coli (E. coli) lacZ (beta-Galactosidase) as a Reporter Gene. Methods Mol Biol 7, 217-235, (1991).

Segall-Shapiro, T. H., Meyer, A. J., Ellington, A. D., Sontag, E. D. & Voigt, C. A. A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol 10, 742, (2014)